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| 产品名称： | MMA |
|---|---|
| 商品货号： | TS211443 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with human adherent mononuclear cells and granulocytes. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human monocyte |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human monocyte |
| Comments： | Animals were immunized with the U-937 histiocytic cell line (ATCC CRL-1593). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with human adherent mononuclear cells and granulocytes. The antibody does not react with circulating T cells, B cells, erythrocytes or platelets. The antibody is cytotoxic. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgM; kappa light chain |
| Name of Depositor： | JF Kearney, H Kubagawa |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Hanjan SN, et al. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin. Immunol. Immunopathol. 23: 172-188, 1982. PubMed: 6179666 |