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微信小章| 产品名称： | MMQ |
|---|---|
| 商品货号： | TS211444 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | pituitary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | tumor |
| Age： | 5 days |
| Applications： | The cells secrete prolactin and express functional dopamine receptors U.S. Patent 5,510,260. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Receptor Expression： | dopamine |
| Genes Expressed： | prolactin |
| Cellular Products： | prolactin |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | The cells secrete prolactin and express functional dopamine receptors U.S. Patent 5,510,260. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 x 105 viable cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3xa0days
Note: The line grows as clumps of cells in suspension. |
| Cryopreservation： | Complete culture medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C Atmosphere: Air, 95%; carbon dioxide (CO2), 5% |
| Population Doubling Time： | 24 to 48 hrs |
| Name of Depositor： | University of Virginia Patent Foundation |
| U.S. Patent Number： | |
| References： | MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996 The MMQ cell line was established from the 7315a rat pituitary tumor. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |