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| 产品名称： | MK-D6 |
|---|---|
| 商品货号： | TS211451 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-A d; cytotoxic |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-A d; cytotoxic |
| Comments： | Mice were immunized with spleen cells from B10.D2 mice. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody does not react with I-Ak, I-Ab, I-As, I-Af or I-Aa. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a; kappa light chain |
| Name of Depositor： | J Kappler, P Marrack |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Kappler JW, et al. Antigen-inducible, H-2 restricted, interleukin-2-producing T cell hybridomas. Lack of independent antigen and H-2 recognition. J. Exp. Med. 153: 1198-1214, 1981. PubMed: 6166712 |