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| 产品名称： | MLH1 |
|---|---|
| 商品货号： | TS211452 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts specifically with the 26000 dalton polypeptide of the chloroplast thylakoid. This antibody and that of MLH2 (ATCC CRL-1779) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Animals were immunized with purified LHC-II. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with purified LHC-II. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 26000 dalton polypeptide of the chloroplast thylakoid. This antibody and that of MLH2 (ATCC CRL-1779) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG1 |
| Name of Depositor： | SC Darr |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Darr SC, et al. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J. Cell Biol. 103: 733-740, 1986. PubMed: 3528171 |