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微信小章| 产品名称： | MLH2 |
|---|---|
| 商品货号： | TS211455 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts specifically with the 28000 dalton polypeptide of the chloroplast thylakoid. This antibody and that of MLH1 (see ATCC CRL-1766) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development. Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified LHC-II. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the light harvesting chlorophyll a/b protein complex (LHC-II) of higher plants |
| Tumorigenic： | Yes |
| Effects： | Yes, in BALB/c mice |
| Comments： | Animals were immunized with purified LHC-II. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 28000 dalton polypeptide of the chloroplast thylakoid. This antibody and that of MLH1 (see ATCC CRL-1766) may be useful in studies of the LHC-II gene family (Cab) and in studying the photoregulation of the Cab genes in chloroplast development. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
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| Subculturing： | Scrape attached cells. Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1 |
| Name of Depositor： | SC Darr |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Darr SC, et al. Monoclonal antibodies to the light-harvesting chlorophyll a/b protein complex of photosystem II. J. Cell Biol. 103: 733-740, 1986. PubMed: 3528171 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |