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| 产品名称： | MLTC-1 |
|---|---|
| 商品货号： | TS211456 |
| Organism： | Mus musculus, mouse |
| Tissue： | testis |
| Cell Type： | Leydig cell |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent, Cells form islands that dome and the monolayer may not become confluent. |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Leydig cell tumor |
| Gender： | male |
| Strain： | C57BL/6 |
| Applications： | This cell line is a suitable transfection host. |
| Karyotype： | polyploid; modal number = 95
Ref  Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Derivation： | This line was established from the M548OP transplantable Leydig cell tumor carried in C57BL/6 mice.Ref Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Clinical Data： | male |
| Receptor Expression： | chorionic gonadotropin; luteinizing hormone (LH) Ref Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Genes Expressed： | progesterone
Ref
Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Cellular Products： | progesterone
Ref Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Tumorigenic： | Yes |
| Effects： | Yes, in C57BL/6 mice |
| Comments： | The cells retain the hormonal responsiveness of the original tumor.
Membrane adenyl cyclase activity is stimulated by treatment with human chorionic gonadotropin (hCG), luteinizing hormone, cholera toxin, sodium fluoride and guanyl-5-ylimidodiphosphate, and produce progesterone in response to hCG. Ref Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Every 3 to 4 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C |
| Population Doubling Time： | 35 to 40 hrs |
| Name of Depositor： | RV Rebois |
| Deposited As： | Mus musculus |
| References： | Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 Rebois RV. Establishment of gonadotropin-responsive murine leydig tumor cell line. J. Cell Biol. 94: 70-76, 1982. PubMed: 6288740 |