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微信小章| 产品名称： | mh mocha |
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| 商品货号： | TS211464 |
| Organism： | Mus musculus, mouse |
| Tissue： | muscle |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Hermansky-Pudlak syndrome (HPS) |
| Age： | 1 to 3 days newborn |
| Storage Conditions： | lliquid nitrogen vapor phase |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: lliquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Name of Depositor： | AA Peden |
| Deposited As： | mouse |
| References： | Peden AA, et al. Assembly and function of AP-3 complexes in cells expressing mutant subunits. J. Cell Biol. 156: 327-336, 2002. PubMed: 11807095 . Mutation in AP-3 delta in the mocha mouse links endosomal transport to storage deficiency in platelets, melanosomes, and synaptic vesicles.. Neuron 21: 111-122, 1998. PubMed: 9697856 The mh cell line was derived from primary fibroblasts taken from muscle of homozygous mocha (mh/mh) mice. As a result of the mocha mutation, the fibroblasts are deficient in the delta subunit of the adaptor-like protein complex, AP-3. The cell line can be used to investigate both the assembly of AP-3 complexes and AP-3 function, and may serve as a model for Hermansky-Pudlak syndrome (HPS), a platelet storage pool deficiency syndrome. |