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微信小章| 产品名称： | MIA PaCa-2 |
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| 商品货号： | TS211465 |
| Organism： | Homo sapiens, human |
| Tissue： | pancreas |
| Cell Type： | epithelial cell |
| Product Format： | frozen |
| Morphology： | attached epithelial with floating rounded cells |
| Culture Properties： | adherent, single cells and loosely attached clusters |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | carcinoma |
| Age： | 65 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent. |
| Images： |  |
| Derivation： | The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male.
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| Clinical Data： | 65 years
Caucasian
male
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| Genes Expressed： | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
| Cellular Products： | human colony stimulating factor, subclass I (CSF-I); plasminogen activator |
| Comments： | The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximately 19%.
Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207 (F1A3-23) and ATCC HB-8208 (F18 AF1).
The line is sensitive to asparaginase. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%; horse serum to a final concentration of 2.5%.
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| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | D5S818: 12,13 D13S317: 12,13 D7S820: 12,13 D16S539: 10,13 vWA: 15 THO1: 9,10 Amelogenin: X TPOX: 9 CSF1PO: 10 |
| Isoenzymes： | G6PD, B |
| Name of Depositor： | A Yunis |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1975 |
| References： | Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590 Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740 Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455 Yunis AA, et al. Human pancreatic carcinoma (MIA PaCa-2) in continuous culture: sensitivity to asparaginase. Int. J. Cancer 19: 128-135, 1977. PubMed: 832918 |
| Cross References： | Nucleotide (GenBank) : M64592 Human macrophage colony stimulating factor (M-CSF-1) mRNA, complete cds. Nucleotide (GenBank) : M76744 Homo sapiens alternatively spliced biliary glycoprotein (BGP) mRNA, 3 end of cds. |