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| 产品名称： | MECA-89 |
|---|---|
| 商品货号： | TS211470 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against murine mucosal vascular addressin (MAdCAM-1) |
| Comments： | Animals were immunized with endothelial cells from mouse mesenteric and peripheral lymph nodes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the second Ig domain of MAdCAM-1, and is a predominant ligand for the lymphocyte mucosal homing receptor (alpha 4, beta 7 integrin heterodimer). Unlike MECA-367, MECA-89 does not inhibit alpha 4, beta 7 dependent binding in vitro. |
| Complete Growth Medium： | Culture Medium: RPMI 1640 with 4.5 g/L glucose, 15 mM HEPES, 2 mM L-glutamine, 0.01 mM non-essential amino acids, and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%. |
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgG2a |
| Name of Depositor： | EC Butcher, DP Andrew |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Streeter PR, et al. A tissue-specific endothelial cell molecule involved in lymphocyte homing. Nature 331: 41-46, 1988. PubMed: 3340147 Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523 Briskin MJ, et al. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363: 461-464, 1993. PubMed: 8502297 |