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微信小章| 产品名称： | MECA-79 |
|---|---|
| 商品货号： | TS211473 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyers patches. It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV). |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse peripheral lymph node endothelial cells (peripheral node addressin) |
| Comments： | Animals were immunized with mouse lymph node stroma. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody specifically blocks lymphocyte adhesion to mouse peripheral lymph node endothelia and does not block lymphocyte binding to mouse Peyers patches. It binds to a sulfate and fucose dependent epitope associated with L-selectin binding ligands of high endothelial venules (HEV). It inhibits L-selectin dependent lymphocyte homing to lymph nodes and blocks lymphocyte binding to HEV in vitro. This line grows best when cultured with a feeder layer of mouse (BALB/c) peritoneal exudate cells. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%; supplemented with 0.05 mM 2-mercaptoethanol. Note: A feeder layer of mouse peritoneal exudate or irradiated murine fibroblasts (e.g. irradiated STO cells, ATCC catalog # X-56) is required for recovery and growth of the MECA-79. |
| Subculturing： | Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgM |
| Name of Depositor： | Stanford University Board of Trustees |
| Deposited As： | rat (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Butcher EC. Method to control leukocyte extravasation. US Patent 5,403,919 dated Apr 4 1995 Berg EL, et al. The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor. J. Cell Biol. 114: 343-349, 1991. PubMed: 1712790 Berg EL, et al. L-selectin-mediated lymphocyte rolling on MAdCAM-1. Nature 366: 695-698, 1993. PubMed: 7505053 Hemmerich S, et al. Sulfation-dependent recognition of high endothelial venules (HEV)- ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody. J. Exp. Med. 180: 2219-2226, 1994. PubMed: 7525849 Michie SA, et al. The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing. Am. J. Pathol. 143: 1688-1698, 1993. PubMed: 8256856 . . Cell. Biol. 107: 1853-1862, 1988. |