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| 产品名称： | MG38 |
|---|---|
| 商品货号： | TS211475 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against the human DEC-205 antigen (human dendritic cell antigen) |
| Comments： | DEC-205-/- knock-out mice were immunized with purified protein from human DEC-205 cells. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody labels mature monocyte-derived dendritic cells but shows weak or no labeling of other peripheral blood mononuclear cells. In tissue sections, MG38 identifies DEC-205 on thymic cortical epithelium and dendritic cells in the thymic medulla and tonsillar T cell areas. The antibody reacts with DEC-205 in tissue sections from both humans and rhesus macaque monkeys. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001.To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%
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| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml. Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Freeze medium: Culture medium with an additional 5% fetal bovine serum, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0°C |
| Isotype： | IgG2b |
| Name of Depositor： | RM Steinman |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Guo M, et al. A monoclonal antibody to the DEC-205 endocytosis receptor on human dendritic cells. Hum. Immunol. 61: 729-738, 2000. PubMed: 10980384 |