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微信小章| 产品名称： | MES-SA/MX2 |
|---|---|
| 商品货号： | TS211476 |
| Organism： | Homo sapiens, human |
| Tissue： | uterus |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | uterine sarcoma |
| Age： | 56 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The line was selected and subcloned in 1988 for resistance to mitoxantrone, an anthracenedione antitumor agent. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | MES-SA/MX2 is a mitoxantrone resistant derivative of the human uterine sarcoma cell line MES-SA (see ATCC CRL-1976). |
| Comments： | These cells were cloned by limiting dilution in soft agar, propagated and tested for sensitively to mitoxantrone. The clone, designated MES-SA/MX2, was approximately 975 fold less sensitive to mitoxantrone than the parental cells. The cells display features of both classic multidrug resistance (MDR), P-glycoprotein overexpression and atypical MDR, altered topoisomerase II catalytic activity. |
| Complete Growth Medium： | A 1:1 mixture of Waymouths MB 752/1 medium and McCoys 5a medium, 90%; fetal bovine serum, 10%
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| STR Profile： | Amelogenin: X CSF1PO: 11 D13S317: 13 D16S539: 11,12 D5S818: 13 D7S820: 7,11 THO1: 6 TPOX: 8,12 vWA: 18 |
| Name of Depositor： | WG Harker |
| Deposited As： | Homo sapiens |
| References： | Harker WG, et al. Development and characterization of a human sarcoma cell line, MES-SA, sensitive to multiple drugs. Cancer Res. 43: 4943-4950, 1983. PubMed: 6883344 Harker WG, Sikic BI. Multidrug (pleiotropic) resistance in doxorubicin-selected variants of the human sarcoma cell line MES-SA. Cancer Res. 45: 4091-4096, 1985. PubMed: 4028002 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |