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微信小章| 产品名称： | MeT-5A |
|---|---|
| 商品货号： | TS211477 |
| Organism： | Homo sapiens, human |
| Tissue： | mesothelium |
| Cell Type： | epithelial virus transformed |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 2 Cells contain polyomavirus DNA sequences
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | pleural fluids obtained from non-cancerous individuals. |
| Applications： | The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned. Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals. |
| Tumorigenic： | No |
| Effects： | No, The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Comments： | The cells stain positively for vimentin, keratins and SV40 T antigen. |
| Complete Growth Medium： | The base medium for this cell line is Medium 199 containing 1.5 g/L sodium bicarbonate.xa0
To make the complete growth medium, add the following components to the base medium:xa0 10% fetal bovine serum (final conc.) 3.3 nM epidermal growth factor (EGF) (final conc.) (do not filter). The trace elements at the following final concentrations:
xa0xa0xa0xa0xa0xa0xa0xa0xa0 This medium is formulated for use with a 5% CO2 in air atmosphere. ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020 (500ml) and ATCC Catalog No. 30-2021 (100ml). |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 11,13 D16S539: 12 D5S818: 12 D7S820: 10 THO1: 6,9.3 TPOX: 8 vWA: 15,18 |
| Name of Depositor： | The United States of America |
| References： | Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989 Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952 |