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| 产品名称： | MDOK |
|---|---|
| 商品货号： | TS211479 |
| Organism： | Ovis aries, sheep |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Virus Susceptibility： | Vesicular stomatitis New Jersey virus
Vesicular stomatitis, Orsay (Indiana) Vesicular stomatitis, Glasgow (Indiana) Infectious bovine rhinotracheitis Bluetongue virus, type 2 |
| Complete Growth Medium： | Minimum essential medium (Eagle) with non-essential amino acids and sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended Medium Renewal: 2 to 3 times per week Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005. |
| Name of Depositor： | RB Owens |
| Deposited As： | Ovis aries |
| References： | Nelson-Rees WA, et al. Debut and accumulation of centric fusion products: an index to age of certain cell lines. Cytogenetics 6: 436-450, 1967. PubMed: 5587551 Madin SH, Darby NB Jr.. Established kidney cell lines of normal adult bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776 Nelson-Rees WA, et al. Peculiarities of ovine cells in culture. Chromosoma 16: 79-89, 1965. PubMed: 14282199 |