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| 产品名称： | ME 1 |
|---|---|
| 商品货号： | TS211482 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27. The antibody cross-reacts with HLA B14 and HLA Bw46. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA B7, Bw22, B27 |
| Comments： | Animals were immunized with the JY human B lymphoblastoid cell line (HLA phenotype A11,A24,B7,B27) and with solubilized HLA A11, A24,B7,B27. Spleen cells were fused with NS-1 myeloma cells. The antibody cross-reacts with HLA B14 and HLA Bw46. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Modified Dulbeccos medium with 0.1 mM hypoxanthine and 0.016 mM thymidine 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5%. |
| Isotype： | IgG1 |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Ellis SA, et al. Recognition of HLA-B27 and related antigen by a monoclonal antibody. Hum. Immunol. 5: 49-59, 1982. PubMed: 6981636 |