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| 产品名称： | MDTC-RP19 |
|---|---|
| 商品货号： | TS211486 |
| Organism： | Meleagris gallopavo, turkey |
| Tissue： | lymph node |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Mareks disease |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Comments： | The line is free of major oncogenic avian viruses. The cells are pathogenic in chickens but fluids are not. The cells grow well at 41C. |
| Complete Growth Medium： | A 1:1 mixture of Leibovitzs L-15 medium and McCoys 5a medium with 2 mM L-glutamine, 55%;
tryptose phosphate broth, 5%;
chicken serum, 20%;
fetal bovine serum, 20%
The fetal bovine serum concentration may be reduced to 10% after the cells have been in culture for 10 to 14 days.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml. |
| Cryopreservation： | Culture medium, 90%; DMSO, 10% |
| Culture Conditions： | Temperature: 41.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | K Nazerian |
| Deposited As： | Meleagris gallopavo |
| U.S. Patent Number： | |
| References： | Nazerian K, et al. Establishment of B-lymphoblastoid cell lines from Mareks disease virus- induced tumors in turkeys. Int. J. Cancer 29: 63-68, 1982. PubMed: 6277804 |