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微信小章| 产品名称： | MDA-MB-175-VII |
|---|---|
| 商品货号： | TS211488 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct; derived from metastatic site: pleural effusion |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 56 years |
| Gender： | female |
| Ethnicity： | Black |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | model number = 84; range = 82 to 89. The cell line is aneuploid female (XX/XXX/XXXX), with chromosome counts in the hypertetraploid to near-tetraploid range. All normal chromosomes are represented by at least one copy, with most chromosomes having three or four copies present per karyotype. Five marker chromosomes are found: del(1)p35), der(7)t(1;7)(q11;q32), unknown, t(2q;?), unknown isochromosome. Several are present in multiple copy. Alterations in the q arm of chromosome N1 are in accordance with the report by M.J. Siciliano, et al., Cancer Res. 39: 919, 1979. |
| Images： |  |
| Derivation： | This is one of a series of breast tumor lines (ATCC HTB-23 through HTB-26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973. |
| Clinical Data： | 56 years Black female |
| Tumorigenic： | Yes |
| Effects： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 7,8 D13S317: 11,12 D16S539: 11,13 D5S818: 11 D7S820: 11,12 THO1: 7 TPOX: 6,8 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 2 PGM3, 1-2 |
| Name of Depositor： | R Cailleau |
| Deposited As： | Homo sapiens |
| References： | Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 |