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微信小章| 产品名称： | MDA-MB-330 |
|---|---|
| 商品货号： | TS211490 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast; Derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Infiltrating lobular carcinoma |
| Age： | 43 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern. |
| Karyotype： | modal number = 64 with one marker chromosome |
| Images： |  |
| Clinical Data： | female Caucasian 43 years |
| Antigen Expression： | Blood Type A; Rh+ |
| Receptor Expression： | epidermal growth factor (EGF) |
| Cellular Products： | tubulin; actin |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Yes, in semisolid medium |
| Comments： | The population exhibited a mixed phenotype when examined by immunofluorescence staining for tubulin, with some cells (type I) having extensive arrays and others (type II) a more diffuse reaction pattern. Most cells of the line stained positively for actin. This line grows very slowly (doubling time is 10 to 20 days) and never becomes confluent. |
| Complete Growth Medium： | These cells are grown in Leibovitzs L-15 medium with 2 mM L-glutamine supplemented with:
(Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Once per week Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution for 1 to 2 minutes, remove trypsin and let the culture sit at 37C until the cells detach. Add fresh medium, aspirate and dispense into new flasks. |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 10 D13S317: 9 D16S539: 9,12 D5S818: 13 D7S820: 10,11 THO1: 8,9.3 TPOX: 8,11 vWA: 14,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 PGM1, 1 PGM3, 1 |
| Population Doubling Time： | 240 to 480 hrs |
| Name of Depositor： | R Cailleau |
| Deposited As： | Homo sapiens |
| References： | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 |