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微信小章| 产品名称： | MDA-MB-134-VI |
|---|---|
| 商品货号： | TS211493 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast/duct; derived from metastatic site: pleural effusion |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | monolayer (lightly adherent) |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | ductal carcinoma |
| Age： | 47 years adult |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | hypodiploid; modal number = 43 with an extra long chromosome 2-like marker; (P28/29) hypodiploid and hypertriploid to hypotetraploid with large subtelocentric marker |
| Images： |  |
| Derivation： | This is one of a series of breast tumor lines (TS211493 through 26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973. |
| Clinical Data： | 47 years adult Caucasian female |
| Antigen Expression： | Antigen expression: Blood Type O; Rh+ |
| Receptor Expression： | Receptor expression: fibroblast growth factor (FGF) |
| Comments： | The cells grow as a monolayer of round loosely adherent cells.
They grow slowly, becoming heavy (shedding somewhat into the medium) without actually becoming confluent.
The cells overexpress FGF receptors, and the fgfr-1 gene is amplified. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Atmosphere: air, 100%
Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 8 D16S539: 13 D5S818: 11,12 D7S820: 10,11 THO1: 6,9 TPOX: 8,11 vWA: 14,15 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | R Cailleau |
| Deposited As： | Homo sapiens |
| References： | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 McLeskey SW, et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994. PubMed: 7506125 Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030 |