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微信小章| 产品名称： | MDA-MB-361 |
|---|---|
| 商品货号： | TS211494 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland/breast; derived from metastatic site:brain |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | adenocarcinoma |
| Age： | 40 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number. = 56; range = 54 to 61. The cell line is aneuploid human female, with chromosome counts in the hyperdiploid range. Normal chromosomes N11 and N17 are absent, chromosomes N1, N20, and N21 are weakly represented, and chromosomes N2, N8, N9, and N15 are single. The remainder of chromosomes are often paired. Eighteen marker chromosomes are found, of which 10 are consistently present. Some of these markers are found to be quite comparable to those described by K.L. Satya-Prakash, et al., in their report on this cell line. |
| Images： |  |
| Clinical Data： | 40 years Caucasian female |
| Oncogene： | wnt7h + |
| Genes Expressed： | Oncogene: wnt7h + |
| Comments： | This line differs from others of the series (ATCC HTB-23,xa0ATCC HTB-24,xa0ATCC HTB-25, andxa0ATCC HTB-26) in karyology and in that it was isolated from a brain metastasis. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Leibovitzs L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%. (Note: The L-15 medium formulation was devised for use in a free gas exchange with atmospheric air. A CO2 and air mixture is detrimental to cells when using this medium for cultivation) |
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 100% |
| STR Profile： | Amelogenin: X CSF1PO: 12 D13S317: 11 D16S539: 11,12 D5S818: 10,11 D7S820: 9,12 THO1: 9.3 TPOX: 8,11 vWA: 17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | R Cailleau |
| Deposited As： | Homo sapiens |
| References： | Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337 Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976. Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779 Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247 Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202 Faust JB, Meeker TC. Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216 Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue. Cancer Res. 54: 2615-2621, 1994. PubMed: 8168088 |