MC-IXC is a twice cloned subline of the neuroepithelioma cell line SK-N-MC (see ATCC HTB-10) which was established in September of 1971 from a metastatic tumor mass.

The choline acetyltransferase activity of MC-IXC was approximately four times higher than the parental line but MC-IXC was negative for dopamine beta hydroxylase activity as was the parental line.

The cells are fibroblast-like and grow to form a dense monolayer.

1. Remove and discard culture medium.
2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53xa0mMxa0EDTA solution to remove all traces of serum that contains trypsin inhibitor.
3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
   Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
5. Add appropriate aliquots of the cell suspension to new culture vessels.
6. Incubate cultures at 37°C

Biedler JL, et al. Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture. Cancer Res. 33: 2643-2652, 1973. PubMed: 4748425

Biedler JL, et al. Multiple neurotransmitter synthesis by human neuroblastoma cell lines and clones. Cancer Res. 38: 3751-3757, 1978. PubMed: 29704