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| 产品名称： | McA-RH8994 |
|---|---|
| 商品货号： | TS211509 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma; Morris hepatoma 8994 |
| Gender： | male |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Receptor Expression： | glucocorticoid |
| Genes Expressed： | alpha-fetoprotein (AFP) |
| Cellular Products： | alpha-fetoprotein (AFP); alpha fetoprotein |
| Comments： | Addition of glucocorticoids induces alpha fetoprotein synthesis and slows cell proliferation. AFP is also synthesized when cell growth slows as the cells end the log phase of growth. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 75%; horse serum, 20%; fetal bovine serum, 5%.
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin solution, remove trypsin immediately and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture once per week.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Every 2 or 3 days |
| Name of Depositor： | JE Becker |
| Deposited As： | Rattus sp. |
| References： | Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974. Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976 |