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微信小章| 产品名称： | McA-RH7777 |
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| 商品货号： | TS211510 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | liver |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | loosely adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | hepatoma; Morris hepatoma 7777 |
| Gender： | female |
| Strain： | Buffalo |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | female |
| Receptor Expression： | glucocorticoid |
| Genes Expressed： | alpha-fetoprotein (AFP, alpha fetoprotein) |
| Cellular Products： | alpha-fetoprotein (AFP, alpha fetoprotein) |
| Comments： | Addition of glucocorticoids (dexamethasone) to the medium accelerates cell proliferation and reduces alpha fetoprotein production. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Heavy monolayer sloughs off; subculture before 70% confluency. Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.xa0
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 weekly is recommended
Medium Renewal: Add medium every 2 to 3 days, do not discard floating cells. |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | JE Becker |
| Deposited As： | Rattus sp. |
| References： | Morris H, Meranze D. Induction and Some Characteristics of “Minimal Deviation” and Other Transplantable Rat Hepatomas. Recent Results Cancer Res. 44: 103-114, 1974. Kulas DT, et al. The transmembrane protein-tyrosine phosphatase LAR modulates signaling by multiple receptor tyrosine kinases. J. Biol. Chem. 271: 748-754, 1996. PubMed: 8557682 Schock D, et al. An auxiliary factor containing a 240-kDa protein complex is involved in apolipoprotein B RNA editing. Proc. Natl. Acad. Sci. USA 93: 1097-1102, 1996. PubMed: 8577721 Becker JE, et al. Two new rat hepatoma cell lines for studying the unbalanced blocked ontogeny hypothesisIn: Becker JE, et al. Onco-developmental gene expression. New YorkAcademic Press, pp. 259-270, 1976 |
| Cross References： | Nucleotide (GenBank) : X56145 Rat mRNA for oncofetal protein (ORF-1A). |