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微信小章| 产品名称： | MC57G |
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| 商品货号： | TS211515 |
| Organism： | Mus musculus, mouse |
| Cell Type： | methylcholanthrene induced |
| Product Format： | frozen |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrosarcoma |
| Strain： | C57BL/6J |
| Applications： | The cell line has been used extensively since 1976 as an H-2b cell line for cell-mediated cytotoxicity assays; the cells express both H-2Kb and H-2Db.xa0 This cell line is the laboratory standard in many laboratories for mouse viral immunity studies.
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| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | The MC57G is a fibrosarcoma cell line established from a tumor arising in a C57BL/6 mouse after treatment with methylcholanthrene. |
| Comments： | The cells express both H-2Kb and H-2Db.xa0 MC57G is infectible by viruses. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for a 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 12 in Culture of Animal Cells: A Manual of Basic Technique by R. Ian Freshney, 4th edition, published by Wiley - Liss, N.Y., 2000. |
| Cryopreservation： | Complete culture medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC® Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | B Knowles |
| Deposited As： | Mus musculus |
| Year of Origin： | 1976 |
| References： | Aden DP, Knowles BB. Cell surface antigens coded by the human chromosome 7. Immunogenetics 3: 209-221, 1976. Trinchieri G, et al. Cell-mediated cytotoxicity to SV40-specific tumour-associated antigens. Nature 261: 312-314, 1976. PubMed: 179019 Doherty PC, et al. H-2 gene expression in required for T cell-mediated lysis of virus-infected target cells. Nature 266: 361-362, 1977. PubMed: 300845 Doherty PC, et al. Cytotoxic T-cell responses in mice infected with influenza and vaccinia viruses vary in magnitude with H-2 genotype. J. Exp. Med. 148: 534-543, 1978. PubMed: 100569 Wiktor TJ, et al. In vitro evidence of cell-mediated immunity after exposure of mice to both live and inactivated rabies virus. Proc. Natl. Acad. Sci. USA 74: 334-338, 1977. PubMed: 299948 This cell line was established from a tumor which developed after treatment with methylcholanthrene. Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |