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| 产品名称： | MCF 10A |
|---|---|
| 商品货号： | TS211517 |
| Organism： | Homo sapiens, human |
| Tissue： | mammary gland; breast |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | fibrocystic disease |
| Age： | 36 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Applications： | This line is suitable as a transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Images： |  |
| Derivation： | MCF 10A was derived from adherent cells in the population. |
| Clinical Data： | 36 years Caucasian female |
| Tumorigenic： | No |
| Effects： | No, in immunosuppressed mice Ref   Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513Yes, in semisolid medium Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 |
| Comments： | The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. The calcium content of the medium exerts a strong effect on the morphology of the cells. |
| Complete Growth Medium： | The base medium for this cell line (MEBM) along with the additives can be obtained from Lonza/Clonetics Corporation as a kit: MEGM, Kit Catalog No. CC-3150. ATCC does not use the GA-1000 (gentamycin-amphotericin B mix) provided with kit.
To make the complete growth medium, you will need to add the following components to the kit (sold separately):
|
| Subculturing： | Remove medium and rinse monolayer with PBS (ATCC® 30-2200™).
Add 3.0 mL 0.05% trypsin, 0.53 mM EDTA and incubate at 37°C for 15 minutes.
To neutralize trypsin, add 3 mL solution of 0.1% soybean trypsin inhibitor (ATCC® 30-2104™).
Centrifuge cell suspension at 125 x g for 5 to 10 minutes.
Resuspend cell pellet in complete culture medium. Add appropriate aliquots of cell suspension to new culture vessels.xa0Corning® T-75 flasks (catalog #430641) are recommended for subculturing this product.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X CSF1PO: 10,12 D13S317: 8,9 D16S539: 11,12 D5S818: 10,13 D7S820: 10,11 THO1: 8,9.3 TPOX: 9,11 vWA: 15,17 |
| Isoenzymes： | AK-1, 1 Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513ES-D, 1 Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513G6PD, B Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513GLO-I, 1-2 Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513PGM1, 1-2 Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513PGM3, 1 Ref Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 |
| Name of Depositor： | Michigan Cancer Foundation |
| Deposited As： | Homo sapiens |
| U.S. Patent Number： | |
| Year of Origin： | August 22, 1984 |
| References： | Soule H, McGrath CM. Immortal human mammary epithelial cell lines. US Patent 5,026,637 dated Jun 25 1991 Pauley RJ, et al. Immortal human mammary epithelial cell sublines. US Patent 5,206,165 dated Apr 27 1993 Soule HD, McGrath CM. A simplified method for passage and long-term growth of human mammary epithelial cells. In Vitro Cell. Dev. Biol. 22: 6-12, 1986. PubMed: 2418007 Soule HD, et al. Isolation and characterization of a spontaneously immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6075-6086, 1990. PubMed: 1975513 Tait L, et al. Ultrastructural and immunocytochemical characterization of an immortalized human breast epithelial cell line, MCF-10. Cancer Res. 50: 6087-6094, 1990. PubMed: 1697506 |