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| 产品名称： | MB4B4 |
|---|---|
| 商品货号： | TS211522 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 Lewis (B cell); BALB/c (myeloma) |
| Applications： | The antibody reacts with all isoforms of mouse CD45. It can be used for immunocytochemistry, flow cytometry, immunoprecipitation and immunoblotting procedures. |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against mouse CD45 (CD45R, CD45RB, T200, common leukocyte antigen) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against mouse CD45 (CD45R, CD45RB, T200, common leukocyte antigen) |
| Comments： | Animals were immunized with anti immunoglobulin activated mouse B lymphoblasts. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with all isoforms of mouse CD45. It can be used for immunocytochemistry, flow cytometry, immunoprecipitation and immunoblotting procedures. |
| Complete Growth Medium： | RPMI 1640 medium with 2 mM L-glutamine, 95%; fetal bovine serum, 5%. The cells can also be grown using 5% fetal bovine serum or in serum free Nutridoma medium
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2a |
| Name of Depositor： | E Pure |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Birkeland ML, et al. Changes in CD45 isoform expression accompany antigen-induced murine T-cell activation. Proc. Natl. Acad. Sci. USA 86: 6734-6738, 1989. PubMed: 2528147 Birkeland ML, et al. Epitopes on CD45R T200 molecules define differentiation antigens on murine B and T lymphocytes. J. Mol. Cell. Immunol. 4: 71-85, 1988. PubMed: 2475124 |