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购买须知
| 产品名称： | MB40.5 |
|---|---|
| 商品货号： | TS211524 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a monomorphic HLA A, B, C specificity and cross-reacts with various primate species. Tested and found negative for ectromelia virus (mousepox). NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state. |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Cellular Products： | immunoglobulin; monoclonal antibody; against HLA A, B, C |
| Comments： | Animals were immunized with papain solubilized HLA B40 antigen. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with a monomorphic HLA A, B, C specificity and cross-reacts with various primate species. The antibody is not cytotoxic. Tested and found negative for ectromelia virus (mousepox). NOTE: a feeder layer of mouse peritoneal macrophages or X-irradiated mouse fibroblasts (see ATCC 56-X, irradiated STO cells) must be used in order to successfully recover these cells from the frozen state. |
| Complete Growth Medium： | Modified Dulbeccos medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG1 |
| Name of Depositor： | P Parham |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Parham P. Monoclonal antibodies against two separate alloantigenic sites of HLA-B40. Immunogenetics 13: 509-527, 1981. PubMed: 7026428 |