微信小陈
微信小章| 产品名称： | MARC S5 |
|---|---|
| 商品货号： | TS211528 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen). High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection. The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. Both ascites and culture media react with extremely high affinity. High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against E. coli O157 O-antigen |
| Cellular Products： | immunoglobulin; monoclonal antibody; against E. coli O157 O-antigen |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in pristane primed BALB/c mice |
| Comments： | Animals were immunized with whole bacterium antigen from E. coli O157:H7 (ATCC 43895). Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen). Both ascites and culture media react with extremely high affinity. High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection. The antibody has been used in ELISA, Western Blot, lateral flow and chemiluminescense assays. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 0.1 mM nonessential amino acids, 80%; heat-inactivated fetal bovine serum, 20%
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal:xa0 Add fresh medium every 2 to 3 days (depending on cell density) |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgM |
| Name of Depositor： | JE Keen |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Westerman RB, et al. Production and characterization of monoclonal antibodies specific for the lipopolysaccharide of Escherichia coli O157. J. Clin. Microbiol. 35: 679-684, 1997. PubMed: 9041412 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online. |