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微信小章| 产品名称： | MB III (de Bruyn-Gey) |
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| 商品货号： | TS211537 |
| Organism： | Mus musculus, mouse |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | monolayer and suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | Tested and found negative for ectromelia virus (mousepox). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Virus Resistance： | poliovirus 3 |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Minimum essential medium (Eagle) in Earles BSS with non-essential amino acids, sodium pyruvate (110 mg/L), 90%; newborn bovine calf serum, 10%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: 2 to 3 times per week Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of flask, aspirate and dispense into new flasks. |
| Culture Conditions： | Atmosphere: Air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| Name of Depositor： | GO Gey |
| Deposited As： | Mus musculus |
| References： | Acta Unio Int. Contra Cancrum 7: 772, 1952. Cancer Res. 9: 282, 1949. Cancer Res. 9: 395, 1949. Von H Owens O, et al. Growth of cells in agitated fluid medium. Ann. N.Y. Acad. Sci. 58: 1039-1055, 1954. PubMed: 14350500 J. Immunol. 76: 475, 1956. J. Exp. Med. 109: 271, 1959. J. Biol. Chem. 234: 1042, 1959. Proc. Soc. Exp. Biol. Med. 100: 686, 1959. De Bruyn WM, Hanson-Melander E. Chromosome studies in the MB mouse lymphosarcoma. J. Natl. Cancer Inst. 28: 1333-1354, 1962. PubMed: 13884145 |