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微信小章| 产品名称： | MAb104 |
|---|---|
| 商品货号： | TS211539 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Animals were immunized with purified nuclei from Xenopus laevis oocytes. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | Immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors) |
| Cellular Products： | Immunoglobulin; monoclonal antibody; against SR proteins (pre-mRNA splicing factors) |
| Comments： | The antibody reacts with a conserved phosphorylated epitope on SR proteins. SR proteins are a family of proteins that have pre-mRNA splicing activity (SR comes from the sequences of consecutive serine and arginine residues in these proteins). The family consists of at least 6 proteins with masses of 75000, 70000, 55000, 40000, 30000 and 20000 daltons. |
| Complete Growth Medium： | Iscoves modified Dulbeccos medium, 80%; fetal bovine serum, 20%
|
| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL. Maintain cultures at a cell concentration between 2 x 105 and 1 x 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Isotype： | IgM |
| Name of Depositor： | MB Roth |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Zahler AM, et al. SR proteins: a conserved family of pre-mRNA splicing factors. Genes Dev. 6: 837-847, 1992. PubMed: 1577277 Roth MB, et al. A conserved family of nuclear phosphoproteins localized to sites of polymerase II transcription. J. Cell Biol. 115: 587-596, 1991. PubMed: 1717489 Zahler AM, et al. Distinct functions of SR proteins in alternative pre-mRNA splicing. Science 260: 219-222, 1993. PubMed: 8385799 Roth MB, et al. A monoclonal antibody that recognizes a phosphorylated epitope stains lampbrush chromosome loops and small granules in the amphibian germinal vesicle. J. Cell Biol. 111: 2217-2223, 1990. PubMed: 1703534 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |