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| 产品名称： | mAb BB7 |
|---|---|
| 商品货号： | TS211540 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody is specific for polypyrimidine tract binding protein (PTB). The antibody may be used in immunodepletion experiments. |
| Derivation： | Spleen cells were fused with Sp2/0 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB) |
| Cellular Products： | immunoglobulin; monoclonal antibody; against polypyrimidine tract binding protein (PTB) |
| Comments： | Animals were immunized with bacterially expressed full length GST-PTB recombinant protein. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody is specific for polypyrimidine tract binding protein (PTB). Polypyrimidine tract binding protein (PTB) is a represssor of pre-mRNA splicing. The antibody may be used in immunodepletion experiments. |
| Complete Growth Medium： | Dulbeccos modified Eagles medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose and supplemented with 10 mM HEPES, 0.1 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
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| Subculturing： | Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2b; kappa light chain |
| Name of Depositor： | DL Black |
| Deposited As： | mouse (B cell); mouse (myeloma) |