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| 产品名称： | Malme-3 |
|---|---|
| 商品货号： | TS211541 |
| Organism： | Homo sapiens, human |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 43 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. Thus, the two lines provide normal and melanoma tumor counterparts for comparative in vitro studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | This is a normal diploid human cell line with 46, XY karyotype and the modal chromosome number of 46 occurring in 78% of cells. No marker chromosomes were detected. Both X and Y chromosomes were single-copied and normal in morphology. |
| Derivation： | This skin fibroblast strain was isolated by J. Fogh from the same patient from whom Malme-3M (ATCC HTB-64) was derived. |
| Clinical Data： | 43 years adult
Caucasian
male
|
| Antigen Expression： | HLA A2, Aw30, B13, B40(+/-), DRw7 |
| Genes Expressed： | HLA A2, Aw30, B13, B40(+/-), DRw7 |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified , Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Interval: Subculture every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Culture medium, 95%; DMSO, 5% |
| Culture Conditions： | Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37°C |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,12 D13S317: 8,13 D16S539: 9,12 D5S818: 11,13 D7S820: 9,12 THO1: 7,8 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. |