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| 产品名称： | Malme-3M |
|---|---|
| 商品货号： | TS211542 |
| Organism： | Homo sapiens, human |
| Tissue： | malignant melanoma;xa0Derived from metastatic site: lung |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | mixed |
| Culture Properties： | mixed adherent-suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Malignant melanoma |
| Age： | 43 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Applications： | This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. The two cell lines provide tumor and normal counterparts for comparativexa0in vitroxa0 melanoma studies. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 88; range = 76 to 93 This is a hypotetraploid human cell line with the modal chromosome number of 88 occurring in 17% of cells. Cells having 82 to 87 chromosome counts were also abundant. Six marker chromosomes were common to most cells, including i(1q), i(6p), i(7q), t(4qter--4pter::?::4q11--4qter) and two others. Except for the paired i(7q), all were single. Two copies of normal X and Y chromosomes were detected in most cells. Normal chromosomes N3 and N17 had five copies in most cells. |
| Images： |  |
| Derivation： | This is one of an extensive series of human tumor lines isolated and characterized by J. Fogh. This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. xad |
| Clinical Data： | Caucasian male This melanoma cell line was isolated from the same patient as Malme-3 (ATCC HTB-102), a normal skin fibroblast. 43 years |
| Antigen Expression： | Antigen expression: HLA A2, Aw30, B13, B40(+/-), DRw7 |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice; forms pigmented malignant melanoma |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Iscoves Modified Dulbeccos Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： |
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Two to three times weekly |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| STR Profile： | Amelogenin: X,Y CSF1PO: 12 D13S317: 8,13 D16S539: 9,12 D5S818: 11 D7S820: 9,12 THO1: 8 TPOX: 8,9 vWA: 15,16 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 1 |
| Name of Depositor： | J Fogh |
| Deposited As： | Homo sapiens |
| References： | Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975. Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871 Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034 Pollack MS, et al. HLA-A, B, C and DR alloantigen expression on forty-six cultured human tumor cell lines. J. Natl. Cancer Inst. 66: 1003-1012, 1981. PubMed: 7017212 |