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| 产品名称： | Mab 108 |
|---|---|
| 商品货号： | TS211550 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain: BALB/c (B cell); BALB/c (myeloma) |
| Applications： | The antibody is specific for the human epidermal growth factor receptor and is capable of inhibiting the growth of human tumor cells expressing human epidermal growth factor receptors. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with NS-1 mouse myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against human epidermal growth factor (EGF) receptor |
| Cellular Products： | immunoglobulin; monoclonal antibody; against human epidermal growth factor (EGF) receptor |
| Comments： | Animals were immunized with CH 71 Chinese hamster ovary cells that had been transfected with a plasmid bearing a truncated form of the epidermal growth factor receptor (EGFR) cDNA. Spleen cells were fused with NS-1 mouse myeloma cells. The antibody is specific for the human epidermal growth factor receptor and is capable of inhibiting the growth of human tumor cells expressing human epidermal growth factor receptors. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium supplemented with 7.5% (v/v) DMSO Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a kappa , IgG2a; kappa light chain |
| Name of Depositor： | Rorer Biotechnology, Inc. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Schlessinger J, et al. Monoclonal antibodies specific to human epidermal growth factor receptor and therapeutic methods employing same. US Patent 6,217,866 dated Apr 17 2001 |