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微信小章| 产品名称： | M5/114.15.2 |
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| 商品货号： | TS211564 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue： | spleen |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Strain： | (BN x Lewis)F1 |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Rats were immunized with a secondary mixed lymphocyte culture (C57BL/6 mouse anti irradiated BN rat lymphoma). Spleen cells were fused with NS-1 myeloma cells.xa0 |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against I-A b, I-A d, I-A q, I-E d and I-E k |
| Cellular Products： | immunoglobulin; monoclonal antibody; against I-A b, I-A d, I-A q, I-E d and I-E k |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105xa0viable cells/mL. Maintain cell density between 5 x 104xa0and 5 x 105xa0viable cells/mL. Medium Renewal: Every 2 to 4 days |
| Cryopreservation： | Complete growth medium supplemented with 5% (v/v) DMSO.xa0Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
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| Culture Conditions： | Temperature: 37°C Atmosphere: 5% CO2 |
| Isotype： | rat IgG2b kappa |
| Name of Depositor： | TA Springer |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Bhattacharya A, et al. A shared alloantigenic determinant on 1a antigens encoded by the I-A and I-E subregions: evidence for 1 region gene duplication. J. Immunol. 127: 2488-2495, 1981. PubMed: 6170707 Davignon D, et al. Lymphocyte function-associated antigen 1 (LFA-1): a surface antigen distinct from Lyt-2,3 that participates in T lymphocyte-mediated killing. Proc. Natl. Acad. Sci. USA 78: 4535-4539, 1981. PubMed: 7027264 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |