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| 产品名称： | M1 |
|---|---|
| 商品货号： | TS211583 |
| Organism： | Mus musculus, mouse |
| Cell Type： | myeloblast |
| Product Format： | frozen |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | myeloid leukemia |
| Strain： | SL |
| Applications： | Can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS and WEHI-3 conditioned medium. |
| Derivation： | Established in vitro from spontaneous myeloid leukemia of SL strain mice. |
| Comments： | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 105 cells/mL and maintain between 1 x 105 and 1 x 106 cells/mL. Medium Renewal: Every 2 to 3 days |
| Name of Depositor： | P Ralph |
| Deposited As： | Mus musculus |
| References： | Ralph P, et al. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J. Immunol. 130: 108-114, 1983. PubMed: 6847876 Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J. Cell. Physiol. 74: 223-234, 1969. PubMed: 4187945 Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022 |