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微信小章| 产品名称： | M/K-2.7 |
|---|---|
| 商品货号： | TS211587 |
| Organism： | Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against murine VCAM-1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against murine VCAM-1 |
| Tumorigenic： | Yes |
| Effects： | Yes, forms ascites in CB.17.SCID mice |
| Comments： | Animals were immunized with stromal cells from mouse bone marrow. Spleen cells were fused with Sp2/0 myeloma cells. The antibody blocks adhesion of lymphocyte precursors to bone marrow stromal cells. The antibody recognizes an antigen believed to be the mouse analog of human VCAM-1 that is expressed on stromal cells in bone marrow and on inflamed endothelial cells (see also M/K-1.9, ATCC CRL-1910). |
| Complete Growth Medium： | RPMI 1640 medium with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
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| Subculturing： | Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 x 105 viable cells/mL.xa0 Maintain cultures at a cell concentration between 0.5 x 105 and 1 x 106 cells/mL. Do not allow the cell concentration to exceed 1 x 106 cells/mL. Medium Renewal: Two to three times weekly |
| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO.xa0 Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Isotype： | IgG1; kappa light chain |
| Name of Depositor： | PW Kincade |
| Deposited As： | rat (B cell); mouse (myeloma) |
| References： | Miyake K, et al. A VCAM-like adhesion molecule on murine bone marrow stromal cells mediates binding of lymphocyte precursors in culture. J. Cell Biol. 114: 557-565, 1991. PubMed: 1713592 Miyake K, et al. Evidence for a role of the integrin VLA-4 in lympho-hemopoiesis. J. Exp. Med. 173: 599-607, 1991. PubMed: 1997648 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |