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微信小章| 产品名称： | M. dunni (Clone III8C) |
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| 商品货号： | TS211595 |
| Organism： | Mus terricolor, Earth-colored mouse |
| Tissue： | skin |
| Cell Type： | fibroblast |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Gender： | female |
| Applications： | This line supports the replication of all four classes of MuLV, i.e., ecotropic, xenotropic, amphotropic and mink cell focus forming (MCF). |
| Storage Conditions： | liquid nitrogen vapor phase |
| Images： |  |
| Derivation： | Clone IIIC8 was derived from tail skin of a normal female Mus terricolor (formally known as Mus dunni) mouse. |
| Virus Susceptibility： | Murine leukemia virus
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| Comments： | Mus terricolor was previously named Mus dunni.xa0 The cells exhibit cytopathic effects when infected with most ecotropic and MCF viruses. They are resistant to infection by Moloney MuLV (presumably due to post translational modifications of the receptor), and lack most endogenous MuLV sequences. To maintain the cells as confluent cultures (in viral assays, for example), use RPMI 1640 or Dulbeccos modified Eagles medium. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated McCoys 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994 |
| Cryopreservation： | Complete growth medium, 95%; DMSO, 5%
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| Culture Conditions： |
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5% |
| Name of Depositor： | M Lander, SK Chattopadhyay Originators: TC Hsu |
| Deposited As： | Mus dunni |
| References： | Lander MR, Chattopadhyay SK. A Mus dunni cell line that lacks sequences closely related to endogenous murine leukemia viruses and can be infected by ectropic, amphotropic, xenotropic, and mink cell focus-forming viruses. J. Virol. 52: 695-698, 1984. PubMed: 6092693 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |