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微信小章| 产品名称： | LS513 |
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| 商品货号： | TS211599 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C,colorectal carcinoma |
| Age： | 63 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | Two stem lines could be distinguished. The main one was represented in 65% of the cells, with a modal number of 51,XY and 3 markers, M1 - der(1)t(1;15), M2 - der(2)t(2;3)der(3)t(2;3), M3, and a monosomy 15., The second stem line had a modal chromosome number of 52,XY and presented M2 and M3 plus an isochromosome for the long arm of chromosome 1 called M4., A trisomy 5,7, a tetrasomy 13, and a monosomy 2 and 3 were present in all of the cells analyzed; the line did not exhibit monosomy 15. |
| Images： |  |
| Derivation： | LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes C mucin secreting cecal tumor located at the Bauhin valve. |
| Clinical Data： | 63 years
Caucasian
male |
| Antigen Expression： | carcinoembryonic antigen (CEA); ICAM-1; HLA class I positive |
| Oncogene： | p53 + (wild type) |
| Genes Expressed： | transforming growth factor beta 1 (TGF beta-1, 83 pg per 106 cells per 24 hours) |
| Cellular Products： | transforming growth factor beta 1 (TGF beta-1, 83 pg per 10 exp6 cells per 24 hours) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | LS513 is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy from a 63 year old Caucasian male patient diagnosed with a Dukes C mucin secreting cecal tumor located at the Bauhin valve.
Approximately 50% of LS513 cells express surface carcinoembryonic antigen (CEA). LS513 cells express the major histocompatibility (MHC) class I antigens HLA and beta 2 microglobulin.
MHC class II antigens (HLA-DR, DQ, and DP were not detected). TGF beta-1 is inhibitory for proliferation of LS513 cells, whereas TGF beta-2 has no effect on the growth of these cells. LS513 cells are 100-fold less sensitive to TGF beta-1 than the LS1034 (ATCC CRL-2158) cell line. LS513 cells are multidrug resistant (MDR) and are tumorigenic in nude mice. Colony forming efficiency was 30% in methylcellulose.
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 to 1:4 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
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| Cryopreservation： | Freeze medium: 50% Culture Medium + 40% FBS + 10% DMSO Storage temperature: liquid nitrogen vapor phase |
| STR Profile： | Amelogenin: X,Y CSF1PO: 10,13 D13S317: 9,10 D16S539: 12,13 D5S818: 9,11 D7S820: 8,11 THO1: 8 TPOX: 8 vWA: 16,17 |
| Population Doubling Time： | 25 hrs |
| Name of Depositor： | L Suardet |
| Deposited As： | Homo sapiens |
| References： | Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643 Burmester JK, et al. Characterization of distinct functional domains of transforming growth factor beta. Proc. Natl. Acad. Sci. USA 90: 8628-8632, 1993. PubMed: 7690965 Li CY, et al. Potential role of WAF1/Cip1/p21 as a mediator of TGF-beta cytoinhibitory effect. J. Biol. Chem. 270: 4971-4974, 1995. PubMed: 7890601 |