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| 产品名称： | LS411N |
|---|---|
| 商品货号： | TS211601 |
| Organism： | Homo sapiens, human |
| Tissue： | cecum |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type B, colorectal carcinoma |
| Age： | 32 years |
| Gender： | male |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 75, X. |
| Derivation： | LS411N is a colorectal carcinoma cell line isolated in 1985 from a primary tumor biopsy of a Dukes B, poorly differentiated cecal carcinoma. The cells obtained after passage in nude mice are referred to as LS411N cells. |
| Clinical Data： | 32 years
Caucasian
male
|
| Antigen Expression： | carcinoembryonic antigen (CEA); ICAM-1; MHC class I positive; MHC Class II (HLA DR, DQ, DP) negative |
| Genes Expressed： | transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours) |
| Cellular Products： | transforming growth factor beta 1 (TGF, 189 pg per 106 cells per 24 hours) |
| Tumorigenic： | Yes |
| Effects： | Yes, forms tumors in nude mice |
| Comments： | The LS411 cell line was found to be contaminated with mycoplasma, and was passed through nude mice to clear the cells of mycoplasma.
A culture submitted to the ATCC in September 1994 was found to be contaminated with mycoplasma, and progeny were cured by a 21 day treatment with BM Cycline.
Approximately 20% of LS411N cells express surface carcinoembryonic antigen (CEA).
The cells express low levels of ICAM-1 HLA class I antigen beta-2-microglobulin.
The cells secrete low levels of latent TGF-beta 1.
TGF-beta 1 and TGF beta 2 are do not inhibit the proliferation of LS411N cells.
Colony forming efficiency was 25% in methylcellulose medium. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subculture Ratio: 1:3 Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994.
|
| Cryopreservation： | Freeze Medium: Complete growth medium, 95%; DMSO, 5%
Storage Temperature: Liquid nitrogen vapor phase |
| Population Doubling Time： | 24 hrs |
| Name of Depositor： | L Suardet |
| Deposited As： | Homo sapiens |
| Passage History： | The cells obtained after passage in nude mice are referred to as LS411N cells. |
| Year of Origin： | 1985 |
| References： | Suardet L, et al. Responsiveness of three newly established human colorectal cancer cell lines to transforming growth factors beta 1 and beta 2. Cancer Res. 52: 3705-3712, 1992. PubMed: 1617643 |