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微信小章| 产品名称： | LS123 |
|---|---|
| 商品货号： | TS211603 |
| Organism： | Homo sapiens, human |
| Tissue： | colon |
| Cell Type： | epithelial |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type B, colorectal adenocarcinoma |
| Age： | 65 years |
| Gender： | female |
| Ethnicity： | Caucasian |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 63; range = 54 to 70. The cells consistently have 18 to 20 marker chromosomes. They are: (t(1;?), del(1)(p13), del(2) (p11), t(2;?), t(7p, 19p), t(8;?), t(11;11) (p15;q13), t(14q,21q), t(6p,16p), t(X;?), and 10-12 others. There is only one copy per cell of structurally unchanged normal chromosomes for Nos. 1,2, 4, 10, 14, 16, and 18. No. 20 generally has 4 copies per cell and the X has 3 copies per cell. No Y chromosome was detected. |
| Images： |  |
| Derivation： | xad
The epithelial cell line, LS123, was established by L.P. Rutzky et al, in 1974 from the second in a series of three primary colonic tumors resected from a 65 year old Caucasian female.xa0
In 1950, a primary colon tumor was resected from the patient (41 years old at that time). 24 years later a second primary tumor classified as a Dukes type B mucin producing colon adenocarcinoma was excised and the cells were cultured. A third primary tumor was discovered in 1980 in an area just proximal to the colostomy and was resected. |
| Clinical Data： | 65 years
Caucasian
female
|
| Genes Expressed： | carcinoembryonic antigen (CEA), 9 ng/mL per 106 cells per 10 days; mucin |
| Cellular Products： | carcinoembryonic antigen (CEA), 9 ng/ml per 10 exp6 cells per 10 days; mucin |
| Tumorigenic： | No |
| Effects： | No, in nude mice (Tumors did not develop within three months in nude mice (5/5) inoculated subcutaneously with 10(7) cells.) |
| Comments： | The established cell line is aneuploid, releases small amounts of carcinoembryonic antigen, fails to grow progressively in nude mice, and forms colonies only in enriched semisolid medium developed for tumor stem cells. LS123 cells grow as confluent cell monolayers in either low serum or serum free medium.
In the chick embryonic skin assay, the cells grow as a well differentiated abnormal colonic epithelium with little mitotic activity and low invasiveness. Although LS123 exhibits several properties associated with neoplasia, they have little invasive potential and may represent an early stage in tumor development.
The plating efficiency is 12% in the medium recommended below. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days |
| Cryopreservation： | Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| STR Profile： | Amelogenin: X CSF1PO: 10,11 D13S317: 9,13 D16S539: 13 D5S818: 11 D7S820: 9 THO1: 7,8 TPOX: 9 vWA: 16,17 |
| Isoenzymes： | AK-1, 1 ES-D, 1 G6PD, B GLO-I, 2 Me-2, 1 PGM1, 1 PGM3, 1-2 |
| Name of Depositor： | LP Rutzky |
| Deposited As： | Homo sapiens |
| Year of Origin： | March, 1974 |
| References： | Rutzky LP, et al. Characterization of a human colonic adenocarcinoma cell line, LS 123. In Vitro 19: 99-107, 1983. PubMed: 6826200 |