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购买须知
| 产品名称： | LP3IIG2 |
|---|---|
| 商品货号： | TS211609 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain： | Strain:xa0 BALB/c (B cell); BALB/c (myeloma) |
| Disclosure： | This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC. |
| Derivation： | Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Legionella pneumophila |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Legionella pneumophila |
| Comments： | Animals were immunized with Legionella pneumophila. Spleen cells were fused with NS-1 myeloma cells. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.
|
| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml. |
| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37.0°C |
| Isotype： | IgG2a |
| Name of Depositor： | Genetic Systems Corp. |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| U.S. Patent Number： | |
| References： | Goldstein LC, Gosting LH. Method and composition for the detection and diagnosis of Legionella pneumophila. US Patent 4,851,333 dated Jul 25 1989 |