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微信小章| 产品名称： | LoVo |
|---|---|
| 商品货号： | TS211611 |
| Organism： | Homo sapiens, human |
| Tissue： | colon; derived from metastatic site: left supraclavicular region |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Dukes type C, grade IV, colorectal adenocarcinoma |
| Age： | 56 years |
| Gender： | male |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | The stemline chromosome number is hyperdiploid with the 2S component occurring at about 2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable. |
| Derivation： | LoVo was initiated in 1971 from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon. |
| Clinical Data： | male |
| Antigen Expression： | HLA A11, B15, B17, Cw1, Cw3; blood type B |
| Oncogene： | myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - |
| Genes Expressed： | carcinoembryonic antigen (CEA) 908 ng/10 6 cells/10 days. xa0The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.xa0 |
| Cellular Products： | carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days |
| Tumorigenic： | Yes |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The cells are negative for expression of CSAp (CSAp-) and colon antigen 3.
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. N-myc and sis oncogene expression were not detected.
Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
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| Cryopreservation： | culture medium 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 95% air, 5% carbon dioxide (CO2) |
| STR Profile： | Amelogenin: XY
CSF1PO: 11,13,14
D13S317: 8, 11
D16S539: 9, 12
D5S818: 11, 12, 13
D7S820: 9.3,10, 11
THO1: 9.3
TPOX: 8,9
vWA: 17,18 |
| Isoenzymes： | ES-D, 1 G6PD, B PGD, A PGM1, 2 PGM3, 1-2 |
| Name of Depositor： | M Romsdahl |
| Deposited As： | Homo sapiens |
| Year of Origin： | 1971 |
| References： | Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641 Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319 Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874 Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905 Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465 Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538 |
| Cross References： | Nucleotide (GenBank) : X17097 Human PSG9 mRNA for pregnancy specific glycoprotein 9. Nucleotide (GenBank) : X17098 Human PSG10 mRNA for pregnancy specific glycoprotein 10. Nucleotide (GenBank) : X14831 Human mRNA for transmembrane carcinoembryonic antigen BGPb (formerly TM2-CEA). |