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| 产品名称： | Lp1 MAB 3 |
|---|---|
| 商品货号： | TS211613 |
| Organism： | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications： | The antibody reacts with a surface antigen on 2 of the 10 antigenic subgroups of L. pneumophila. and it is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays. Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed： | immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1 |
| Cellular Products： | immunoglobulin; monoclonal antibody; against Legionella pneumophila serogroup 1 |
| Comments： | Animals were immunized with the bacterial antigenic subgroup Knoxville 1 (ATCC 33153). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with a surface antigen on 2 of the 10 antigenic subgroups of L. pneumophila. and it is useful in clinical diagnostic tests for subtyping L. pneumophila serogroup 1 isolates for epidemiologic studies using indirect immunofluorescence assays. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
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| Subculturing： | Medium Renewal: Every 2 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype： | IgG2b |
| Name of Depositor： | RM McKinney |
| Deposited As： | mouse (B cell); mouse (myeloma) |
| References： | Joly JR, et al. Development of a standard subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J. Clin. Microbiol. 23: 768-771, 1986. PubMed: 3517064 McKinney RM, et al. Monoclonal antibodies to Legionella pneumophila serogroup 1: possible applications in diagnostic tests and epidemiologic studies. Zentralbl. Bakteriol. Mikrobiol. Hyg. I Abt. Orig. A255: 91-95, 1983. |