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微信小章| 产品名称： | L-M(TK-) LM(tk-), LMTK- |
|---|---|
| 商品货号： | TS211621 |
| Organism： | Mus musculus, mouse |
| Tissue： | subcutaneous connective tissue; areolar and adipose |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | 100 days |
| Gender： | male |
| Strain： | C3H/An |
| Applications： | This cell line is a suitable transfection host. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 45; range = 42 to 48. The stemline chromosome number is hyperdiploid with the 2S component occurring at 3.8%. Twenty-one markers were common to nearly all S metaphases studied. Of these, 13 were two-armed chromosomes, and eight, acrocentric. Four markers were isochromosomes by Robertsonian translocations from chromosomes 4, 12, 14 and 15. Normal X, 12, 14, 15 and 16 were absent. The X chromosome was present as an arm of Rb(X.3). (See Cancer Genet. Cytogenet. 24: 95-107, 1987 for a detailed karyotype description.) Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines. |
| Clinical Data： | male |
| Antigen Expression： | H-2k |
| Tumorigenic： | Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10exp7 cells |
| Effects： | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments： | The cells are 5-bromo-2-deoxyuridine resistant (BUDR, BrdUrd) and thymidine kinase (TK) deficient, HAT sensitive. Tested and found negative for ectromelia virus (mousepox).
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| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week |
| Cryopreservation： | Freeze medium: complete growth medium, 95%; DMSO, 5% |
| Culture Conditions： | Temperature: 37°C
Atmosphere:xa0 air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor： | TR Chen |
| Deposited As： | Mus musculus |
| References： | Kit S, et al. Deletion of thymidine kinase activity from L cells resistant to bromodeoxyuridine. Exp. Cell Res. 31: 297-312, 1963. PubMed: 14056989 Chen TR. Cytogenetics of somatic cell hybrids. II. Modal karyotypes and mosaicism in mouse mutant cell lines: LM(tk-), A9, and Rag. Cancer Genet. Cytogenet. 24: 95-107, 1987. PubMed: 3466673 Hsu TC, Somers CE. Properties of L cells resistant to 5-bromodeoxyuridine. Exp. Cell Res. 26: 404-410, 1962. PubMed: 14449548 Boone C, et al. Assignment of three human genes to chromosomes (LDH-A to 11, TK to 17, and IDH to 20) and evidence for translocation between human and mouse chromosomes in somatic cell hybrids (thymidine kinase-lactate dehydrogenase A-isocitrate dehydrogenase-C-11, E-17, and F-20 chromosomes). Proc. Natl. Acad. Sci. USA 69: 510-514, 1972. PubMed: 4110482 Peterson JL, McBride OW. Cotransfer of linked eukaryotic genes and efficient transfer of hypoxanthine phosphoribosyltransferase by DNA-mediated gene transfer. Proc. Natl. Acad. Sci. USA 77: 1583-1587, 1980. PubMed: 6929511 Higazi AA, et al. Soluble human urokinase receptor is composed of two active units. J. Biol. Chem. 272: 5348-5353, 1997. PubMed: 9030610 Carballo E, et al. Bone marrow transplantation reproduces the tristetraprolin-deficiency syndrome in recombination activating gene-2 (-/-) mice. J. Clin. Invest. 100: 986-995, 1997. PubMed: 9276715 |