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微信小章| 产品名称： | L-M |
|---|---|
| 商品货号： | TS211622 |
| Organism： | Mus musculus, mouse |
| Tissue： | subcutaneous connective tissue; areolar and adipose |
| Product Format： | frozen |
| Morphology： | fibroblast |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | normal |
| Age： | 100 days |
| Gender： | male |
| Strain： | C3H/An |
| Storage Conditions： | liquid nitrogen vapor phase |
| Clinical Data： | male |
| Antigen Expression： | H-2k |
| Virus Resistance： | poliovirus 1, 2, 3 |
| Comments： | Grows in protein free medium. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | Medium 199 with Earles BSS supplemented with 0.5% Bacto-peptone
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| Subculturing： | Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells from the floor of the flask, aspirate and dispense into new flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
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| Cryopreservation： | Complete growth medium described above supplemented with 5% (v/v) DMSO. Cell culture tested DMSO is available as ATCC Catalog No. 4-X. |
| Culture Conditions： | Temperature: 37°C
Atmosphere: 5% CO2 |
| Name of Depositor： | DJ Merchant |
| Deposited As： | Mus musculus |
| References： | Kuchler RJ, Merchant DJ. Propagation of strain L (Earle) cells in agitated fluid suspension cultures. Proc. Soc. Exp. Biol. Med. 92: 803-806, 1956. PubMed: 13370534 Munyon WH, Merchant DJ. The relation between glucose utilization, lactic acid production and utilization and the growth cycle of L strain fibroblasts. Exp. Cell Res. 17: 490-498, 1959. PubMed: 13672205 Merchant DJ, Hellman KB. Growth of L-M strain mouse cells in a chemically defined medium. Proc. Soc. Exp. Biol. Med. 110: 194-198, 1962. PubMed: 14472756 Hsu TC, Merchant DJ. Mammalian chromosomes in vitro. XIV. Genotypic replacement in cell populations. J. Natl. Cancer Inst. 26: 1075-1083, 1961. PubMed: 13716385 Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC. Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988. Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC. |