微信小陈
微信小章
产品简介
购买须知
| 产品名称： | LLC-MK2 Derivative |
|---|---|
| 商品货号： | TS211630 |
| Organism： | Macaca mulatta, monkey, rhesus |
| Tissue： | kidney |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age： | adult |
| Storage Conditions： | liquid nitrogen vapor phase |
| Karyotype： | modal number = 70; range = 41 to 136. No marker chromosomes noted.No marker chromosomes noted. |
| Genes Expressed： | plasminogen activator; keratin. |
| Cellular Products： | plasminogen activator; keratin |
| Virus Susceptibility： | Human poliovirus 1
Human poliovirus 2 Human poliovirus 3 |
| Comments： | The cells are positive for keratin by immunoperoxidase staining. |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Eagles Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing： | Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation： | Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions： | Temperature: 37°C |
| Name of Depositor： | RN Hull |
| Deposited As： | Macaca mulatta |
| References： | Holscher C, et al. Defective nitric oxide effector functions lead to extreme susceptibility of Trypanosoma cruzi-infected mice deficient in gamma interferon receptor or inducible nitric oxide synthase. Infect. Immun. 66: 1208-1215, 1998. PubMed: 9488415 |