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微信小章| 产品名称： | LLC-WRC 256 |
|---|---|
| 商品货号： | TS211637 |
| Organism： | Rattus norvegicus, rat |
| Tissue： | mammary gland |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | adherent |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | mammary gland; Walker carcinoma |
| Strain： | Walker |
| Tumorigenic： | Yes |
| Effects： | Yes, in Amsterdam rats |
| Virus Resistance： | poliovirus 1 |
| Complete Growth Medium： | Medium 199, 95%; horse serum, 5%
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| Subculturing： | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin for 1 to 2 minutes, remove trypsin and let culture sit at room temperature for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. |
| Name of Depositor： | RN Hull |
| Deposited As： | Rattus norvegicus |
| References： | American Association of Anatomists. Sixty-ninth annual session; Marquette University School of Medicine, Milwaukee, Wisconsin. Anat. Rec. 124: 490, 1956. Hull RN. Establishing long-term cultures of mammalian normal, solid tumor, and ascites tumor cells on glass. Science 117:223, 1953. Pubmed : 13038481 Hull RN, Cherry WR, Johnson IS. The adaptation and maintenance of mammalian cells to continuous growth in tissue culture. Anat Rec 124: 490–499,1956. Earle WR. A study of the Walker rat mammary carcinoma 256, in vivo and in vitro. Am J Cancer 24:566-612, 1935. |