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| 产品名称： | Lec2 originally named Pro-5WgaRII6A |
|---|---|
| 商品货号： | TS211639 |
| Organism： | Cricetulus griseus, hamster, Chinese |
| Tissue： | ovary |
| Product Format： | frozen |
| Morphology： | epithelial |
| Culture Properties： | monolayer or suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Gender： | female |
| Applications： | This cell linexa0exhibits a drastic reduction in the transport of CMP-sialic acid into the Golgi compartment, and may be useful for studying the role of sialic acid in the function and compartmentalization of glycosylated macromolecules. |
| Storage Conditions： | liquid nitrogen vapor phase |
| Derivation： | This line is a mutant clone derived from the parental CHO clone Pro-5 (a proline auxotroph, see ATCC CRL-1781) by selection for resistance to wheat germ agglutinin. |
| Clinical Data： | female |
| Comments： | The cells are proline auxotrophs and must be grown in a medium that contains proline (40 mg/L).
The population contains Pro+ revertants at high frequency (approximately 1 in 25 cells), and should be cloned if it is desired to use the Pro- marker in complementation studies. |
| Complete Growth Medium： | Alpha minimum essential medium, 90%; fetal bovine serum, 10%
|
| Subculturing： | Remove medium, add fresh 0.25% trypsin, let sit for 2 to 3 minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Name of Depositor： | P Stanley |
| Deposited As： | Cricetulus griseus |
| References： | Deutscher SL, et al. Translocation across Golgi vesicle membranes: a CHO glycosylation mutant deficient in CMP-sialic acid transport. Cell 39: 295-299, 1984. PubMed: 6498937 Stanley P, Siminovitch L. Complementation between mutants of CHO cells resistant to a variety of plant lectins. Somatic Cell Genet. 3: 391-405, 1977. PubMed: 601679 |