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| 产品名称： | LK 35.2 |
|---|---|
| 商品货号： | TS211641 |
| Organism： | Mus musculus (B cell); Mus musculus (lymphoma), mouse (B cell); mouse (lymphoma) |
| Cell Type： | hybridoma: B lymphocyte |
| Product Format： | frozen |
| Morphology： | lymphoblast |
| Culture Properties： | suspension |
| Biosafety Level： | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease： | Lymphoma |
| Applications： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.BR mice (I-Ak, I-Ek). Tested and found negative for ectromelia virus (mousepox). |
| Derivation： | The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.BR mice (I-Ak, I-Ek). |
| Comments： | The LK 35.2 bears surface I-Ad,k and I-Ed,k molecules and can present antigen to appropriate I region restricted T cell hybridomas. The line was produced by fusing A20.2J lymphoma cells (I-Ad, I-Ed) with T cell depleted spleen cells from B10.BR mice (I-Ak, I-Ek). Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium： | The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing： | Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 4 X 10 exp5 cells/ml and maintain between 3 X 10 exp5 and 2 X 10 exp6 cells/ml. |
| Name of Depositor： | P Marrack, J Kappler |
| Deposited As： | mouse (B cell); mouse (lymphoma) |
| References： | Kappler J, et al. Antigen presentation by Ia+ B cell hybridomas to H-2 restricted T cell hybridomas. Proc. Natl. Acad. Sci. USA 79: 3604-3607, 1982. PubMed: 6980413 |